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What is the biological value of different protein sources?

The biological value of proteins determines how well your body can use them for muscle building and recovery. This value varies greatly between different protein sources, with animal proteins generally scoring higher than plant-based alternatives. For supplement manufacturers, this is an important factor when choosing the right protein source for their products.

In this article, we answer all the important questions about biological value and help you understand which protein sources perform best. We also look at modern alternatives and what this means for supplement formulation.

What is biological value and why is it important with proteins?

Biological value measures how efficiently your body can use a protein to make body protein. The scale runs from 0 to 100, where 100 means that all absorbed amino acids are fully utilised by the body.

This value depends on the amino acid profile of the protein. Proteins with all nine essential amino acids in the right proportions have a higher biological value. Your body can better use these proteins for muscle building, recovery and other important processes.

This is especially relevant for athletes and active people, as they need more protein for muscle recovery. A protein with a high biological value ensures that your body has more of the same amount of protein. This can make the difference between an effective supplement and one that performs less well.

Which protein sources have the highest biological value?

Egg protein ranks first with a biological value of 100, followed by whey protein (104-110), casein (77) and beef (80). These animal proteins contain all the essential amino acids in optimal proportions.

Plant proteins score lower on this scale. Soy protein achieves around 74, pea protein around 65 and rice protein around 57. These lower scores are because plant proteins are often deficient in certain essential amino acids, especially lysine or methionine.

Modern supplement manufacturers therefore combine different plant proteins to address these deficiencies. For example, a blend of pea and rice protein can achieve a much higher biological value than each of these proteins individually.

How does biological value differ between animal and plant proteins?

Animal proteins have an average biological value of 70-110, while plant proteins usually score between 45 and 75. This difference arises because animal proteins have a more complete amino acid profile that better suits the needs of the human body.

The main difference is in the presence of all essential amino acids. Animal proteins almost always contain them in sufficient quantities, while vegetable proteins often have limiting amino acids. Rice protein, for instance, is low in lysine, while pea protein is deficient in methionine.

This does not mean that plant-based proteins are bad. Through clever combinations, manufacturers can create plant-based blends that are close to animal proteins in terms of biological value. Moreover, plant proteins have other advantages, such as better digestibility for some people and lower environmental impact.

What makes whey protein so valuable for supplements?

Whey protein has a biological value of 104-110 and is absorbed by the body very quickly, often within 30-60 minutes of ingestion. This makes it ideal for post-workout supplements where fast muscle recovery is desired.

Its high biological value comes from whey's excellent amino acid profile. It is high in leucine, an amino acid that directly stimulates muscle protein synthesis. In addition, whey contains all the essential amino acids in optimal proportions for the human body.

Whey is also technically an excellent raw material for supplements. It dissolves well, has a neutral taste that is easy to mask and remains stable during storage. These properties make it a favourite choice for manufacturers looking to make high-quality protein products.

How does processing affect the biological value of protein sources?

Processing can both increase and decrease the biological value of proteins, depending on the methods and temperatures used. Mild processing techniques usually preserve the original value, while aggressive treatment can reduce it.

Heat is an important factor. Too high temperatures can damage amino acids or alter the protein structure in such a way that the body is less able to utilise it. This is why high-quality producers often use cold extraction methods or low temperatures during processing.

With plant proteins, processing can actually help. By removing certain anti-nutrients or optimising the protein structure, manufacturers can increase the biological value. Combining different plant proteins during processing can also improve the overall biological value of the final product.

What factors determine the choice of protein sources in supplement formulation?

The choice of protein sources depends on several factors: the product's target audience, desired biological value, cost, flavour profile, solubility and any dietary restrictions, such as veganism or lactose intolerance.

For sports products, manufacturers often choose whey because of its high biological value and rapid absorption. For vegan products, plant-based blends are necessary, with the trick being to create a combination that is as close as possible to animal proteins in terms of biological value.

Practical considerations also come into play. Some proteins dissolve better, others have a more neutral taste and still others are more stable during storage. An experienced formulator weighs all these factors against each other to make the best product for the specific application.

We at MixMasters understand this complexity well. Whether you are looking for traditional whey solutions or modern plant-based alternatives, our R&D team will help you choose the right protein source for your specific product. We can test and optimise different options to find the perfect balance between biological value, taste and functionality. See our comprehensive service to see how we can support your supplement formulation.

Frequently Asked Questions

As a manufacturer, how can I optimise the biological value of plant protein blends?

Combine complementary plant proteins such as pea and rice protein in a ratio of about 70:30. Add amino acids that are deficient, such as lysine to rice protein or methionine to pea protein. Test different ratios and have the amino acid profiles analysed to achieve the optimal biological value.

What are the cost-benefit trade-offs between whey and plant-based protein sources?

Whey is usually more expensive but offers higher biological value and better functionality. Plant-based proteins are often cheaper per kilo, but you need more for the same effect. Plant-based blends can be cost-effective if you find the right combination that comes close to whey in terms of performance.

How do I test whether my protein formulation actually has high biological value?

Have an amino acid analysis performed to determine the complete profile. Calculate the PDCAAS score (Protein Digestibility Corrected Amino Acid Score) or ask a specialised lab to measure the biological value. Consumer feedback on efficacy and digestibility also provides valuable insights.

Which processing errors reduce biological value the most?

Too high temperatures (above 80°C) damage hite-sensitive amino acids such as lysine and tryptophan. Prolonged exposure to acid or base during extraction can also damage protein structures. Always use mild processing methods and control temperature during all process steps.

Can I increase the biological value by adding enzymes to my formulation?

Yes, digestive enzymes such as protease can improve protein digestion and thus increase bioavailability. Add these enzymes in the right dosage (usually 50-100mg per serving) and make sure they remain stable during storage. This is especially effective with plant-based proteins.

How do I effectively communicate biological value to consumers?

Gebruik begrijpelijke termen zoals ‘complete eiwitten’ of ‘alle essentiële aminozuren’ in plaats van technische waarden. Vergelijk je product met bekende bronnen (‘net zo compleet als ei-eiwit’) en leg uit wat dit betekent voor hun doelen. Focus op de praktische voordelen zoals betere spierherstel of efficiëntere eiwitbenutting.

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Meal shakes:

Ingredients: wholemeal oatmeal (gluten), whey protein concentrate (MILK), sunflower oil powder (sunflower oil, modified starch, glucose syrup, silicon dioxide, natural flavouring), maltodextrin, isomaltulose, vitamin and mineral blend (tri-calcium citrate, di-calcium phosphate, potassium chloride, maltodextrin, magnesium oxide, di-calcium phosphate, sodium ascorbate, iron pyrophosphate, tocopheryl acetate, zinc sulphate, folic acid, potassium iodide, niacin, sodium molybdate, sodium selenite, retinyl acetate, vitamin K1, calcium D-pantothenate, manganese sulphate, d-biotin, chromium III chloride, copper sulphate, cyanocobalamin, ergocalciferol, pyridoxine hydrochloride, riboflavin, thiamine mononitrate), inulin, thickener: xanthan gum, emulsifier: sunflower lecithin, sweetener: sucralose

NUTRITIONAL VALUE

 
 

ENERGETIC VALUE

Kilojoule

1669,98

KJ/100g

2170,97

KJ/130g

 

Kilo calorie

397,60

Kcal/100g

516,88

Kcal/130g

 

FATS

total

12,38

g/100g

16,09

g/130g

 

saturated

1,75

g/100g

2,27

g/130g

 

COAL HYDRATES

total

45,35

g/100g

58,96

g/130g

 

sugar

5,92

g/100g

7,69

g/130g

 

EGG WHITES

total

23,12

g/100g

30,05

g/130g

 

FOOD FEE

total

7,58

g/100g

9,85

g/130g

 

Energy Booster:

INGREDIENTS WITH PHYSIOLOGICAL OPERATION PER PORTION OF 10 g

Taurine

2000 mg

L-tyrosine

1000 mg

L-Carnitine       

400 mg

Maca powder

250 mg

Caffeine

200 mg

Black pepper extract    

100 mg

Magnesium

99 mg

Vitamin C       

80 mg

Vitamin B3 (Nicotinamide)

16 mg

Vitamin B6 (Pyridoxine)

1 mg

Cyanocobalamin (Vit B12)

2.5 µg

Vegetable protein shakes (vegan): Ingredients: pea protein isolate, flavouring, sweetener: sucralose
NUTRITIONAL VALUE
ENERGETIC VALUE Kilojoule 1653,97 KJ/100g 496,19 KJ/30g
Kilo calorie 395,44 Kcal/100g 118,63 Kcal/30g
FATS total 7,90 g/100g 2,37 g/30g
saturated 2,03 g/100g 0,61 g/30g
COAL HYDRATES total 2,21 g/100g 0,66 g/30g
sugar 1,18 g/100g 0,36 g/30g
EGG WHITES total 84,77 g/100g 25,43 g/30g
FOOD FEE total 8,90 g/100g 2,67 g/30g

Whey protein isolate shakes c.a. 90% protein:

Ingredients: whey protein isolate (MELK), thickener: xanthan gum, emulsifier: sunflower lecithin, sweetener: sucralose

 

NUTRITIONAL VALUE

 
 

ENERGETIC VALUE

Kilojoule

1528,87

KJ/100g

458,66

KJ/30g

 

Kilo calorie

359,97

Kcal/100g

107,99

Kcal/30g

 

FATS

total

0,29

g/100g

0,09

g/30g

 

saturated

0,24

g/100g

0,07

g/30g

 

COAL HYDRATES

total

2,56

g/100g

0,77

g/30g

 

sugar

2,56

g/100g

0,77

g/30g

 

EGG WHITES

total

89,01

g/100g

26,70

g/30g

 

FOOD FEE

total

0,73

g/100g

0,22

g/30g

 
Whey protein concentrate shakes c.a. 75% protein: Ingredients: whey protein concentrate (MELK), thickener: xanthan gum, emulsifier: sunflower lecithin, sweetener: sucralose
NUTRITIONAL VALUE
ENERGETIC VALUE Kilojoule 1621,80 KJ/100g 486,54 KJ/30g
Kilo calorie 383,44 Kcal/100g 115,03 Kcal/30g
FATS total 6,85 g/100g 2,05 g/30g
saturated 2,93 g/100g 0,88 g/30g
COAL HYDRATES total 4,03 g/100g 1,21 g/30g
sugar 4,03 g/100g 1,21 g/30g
EGG WHITES total 80,21 g/100g 24,06 g/30g
FOOD FEE total 0,73 g/100g 0,22 g/30g

Meal shakes vegetable (vegan):

Ingredients: whole grain oatmeal (gluten), pea protein isolate, sunflower oil powder, (sunflower oil, modified starch, glucose syrup, silicon dioxide, natural flavouring), maltodextrin, isomaltulose, vitamin and mineral blend (tri-potassium citrate, di-potassium phosphate, potassium chloride, maltodextrin, magnesium oxide, di-potassium phosphate, sodium ascorbate, iron pyrophosphate, tocopheryl acetate, zinc sulphate, folic acid, potassium iodide, niacin, sodium molybdate, sodium selenite, retinyl acetate, vitamin K1, calcium D-pantothenate, manganese sulphate, d-biotin, chromium III chloride, copper sulphate, cyanocobalamin, ergocalciferol, pyridoxine hydrochloride, riboflavin, thiamine mononitrate), inulin, thickener: xanthan gum, sweetener: sucralose

NUTRITIONAL VALUE

 
 

ENERGETIC VALUE

Kilojoule

1665,91

KJ/100g

2165,68

KJ/130g

 

Kilo calorie

397,45

Kcal/100g

516,69

Kcal/130g

 

FATS

total

12,56

g/100g

16,33

g/130g

 

saturated

1,57

g/100g

2,03

g/130g

 

COAL HYDRATES

total

44,70

g/100g

58,11

g/130g

 

sugar

5,15

g/100g

6,70

g/130g

 

EGG WHITES

total

23,86

g/100g

31,02

g/130g

 

FOOD FEE

total

9,29

g/100g

12,08

g/130g

 
Fat-burner:
INGREDIENTS WITH PHYSIOLOGICAL OPERATION PER PORTION OF 7 g
L-tyrosine 1200 mg
L-Carnitine 1000 mg
Gamma-Aminobutyric Acid (GABA) 300 mg
Green tea extract 300 mg
Choline bitartrate 250 mg
Guarana 100 mg
Caffeine 100 mg
EGCG (from green tea extract) 45 mg

Pre-workout:

INGREDIENTS WITH PHYSIOLOGICAL OPERATION PER PORTION OF 10 g

L-citrulline DL-malate 2:1

2000 mg

Beta alanine

1600 mg

Taurine

1200 mg

L-tyrosine

1000 mg

Caffeine

250 mg

Flavours:

  • Strawberry cheesecake
  • Strawberry natural
  • Pineapple
  • Pineapple natural
  • Apple
  • Apple natural
  • Apple cake
  • Banana of course
  • Bitter almond natural
  • Blueberry
  • Blueberry natural
  • Forest fruit natural
  • Cappuccino
  • Cheesecake Naturally
  • Chocolate Natural
  • Cola of course
  • Cookie & Cream
  • Grape
  • Curd blueberry
  • Raspberry natural
  • Roasted apple
  • Green tea
  • Hazelnut
  • Honey
  • Caramel natural
  • Cherries, of course
  • Kiwi
  • Coconut natural
  • Mango of course
  • Natural lemon aroma
  • Natural citrus aroma
  • Pannacotta
  • Passion fruit
  • Pear of course
  • Peach
  • Peanut butter
  • Pistachio
  • Plusvanilla
  • Rice
  • Room
  • Cream of course
  • Orange natural
  • Tiramisu
  • Tomato mozzarella
  • Vanilla natural
  • Vanillin
  • Plus vanilla of course
  • Walnut
  • Watermelon
  • Watermelon of course
  • White chocolate of course

Combinations are possible